What is the purpose of the AHG phase?

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Multiple Choice

What is the purpose of the AHG phase?

The AHG phase is about finding antibodies that have already attached to red cells. After the incubation and washing steps, the antiglobulin reagent (anti-human globulin) is added to bridge or detect whatever is bound on the cell surface. This reveals sensitized cells that would not agglutinate on their own, highlighting antibodies that are most likely to cause trouble in a transfusion. The reason this option fits best is that the AHG phase is aimed at identifying antibodies that are bound to red cells and are clinically significant—primarily IgG antibodies, and in some cases antibodies that have activated complement on the cell surface. These are the antibodies we worry about when matching blood for transfusion because they can cause hemolytic reactions. While IgM antibodies often react at earlier testing steps, the AHG phase is the one that demonstrates antibodies that have bound to cells and have real transfusion relevance, including those that are IgM or IgG if they are bound in a way that makes them clinically significant.

The AHG phase is about finding antibodies that have already attached to red cells. After the incubation and washing steps, the antiglobulin reagent (anti-human globulin) is added to bridge or detect whatever is bound on the cell surface. This reveals sensitized cells that would not agglutinate on their own, highlighting antibodies that are most likely to cause trouble in a transfusion.

The reason this option fits best is that the AHG phase is aimed at identifying antibodies that are bound to red cells and are clinically significant—primarily IgG antibodies, and in some cases antibodies that have activated complement on the cell surface. These are the antibodies we worry about when matching blood for transfusion because they can cause hemolytic reactions. While IgM antibodies often react at earlier testing steps, the AHG phase is the one that demonstrates antibodies that have bound to cells and have real transfusion relevance, including those that are IgM or IgG if they are bound in a way that makes them clinically significant.

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