What would be the best positive control for anti-C?

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Multiple Choice

What would be the best positive control for anti-C?

Positive controls in antibody testing must use red cells that carry the antigen the antibody is being tested against. For anti-C, you need cells that express the C antigen so the antibody can bind and produce a reaction if present. A cell line that is heterozygous for C (has C antigen on the red cell surface) provides a reliable positive signal, confirming that the test system is working and that anti-C can be detected. The rr option lacks C antigen, so it would not produce a reaction and isn’t useful as a positive control. Cells that are homozygous for C (R1R1 or R2R2) also express C and could serve as positive controls, but a heterozygous C cell offers a representative level of antigen density that’s dependable across different reagents and conditions, making it the best choice for a positive control.

Positive controls in antibody testing must use red cells that carry the antigen the antibody is being tested against. For anti-C, you need cells that express the C antigen so the antibody can bind and produce a reaction if present. A cell line that is heterozygous for C (has C antigen on the red cell surface) provides a reliable positive signal, confirming that the test system is working and that anti-C can be detected.

The rr option lacks C antigen, so it would not produce a reaction and isn’t useful as a positive control. Cells that are homozygous for C (R1R1 or R2R2) also express C and could serve as positive controls, but a heterozygous C cell offers a representative level of antigen density that’s dependable across different reagents and conditions, making it the best choice for a positive control.

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