What would be the best negative control for anti-C?

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Multiple Choice

What would be the best negative control for anti-C?

Anti-C will only react with red cells that carry the C antigen. A negative control must lack that antigen so any reaction observed can be attributed to non-specific factors rather than anti-C binding. An RBCs genotype rr, which is homozygous without C antigen, provides exactly that: no C antigen means no agglutination should occur with anti-C, establishing a true baseline. In contrast, cells that express C antigen would show reactivity if anti-C is present, and cells homozygous for C would certainly react, so they wouldn’t serve as a negative control.

Anti-C will only react with red cells that carry the C antigen. A negative control must lack that antigen so any reaction observed can be attributed to non-specific factors rather than anti-C binding. An RBCs genotype rr, which is homozygous without C antigen, provides exactly that: no C antigen means no agglutination should occur with anti-C, establishing a true baseline. In contrast, cells that express C antigen would show reactivity if anti-C is present, and cells homozygous for C would certainly react, so they wouldn’t serve as a negative control.

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