Which technique is used to reveal true ABO grouping in the presence of cold autoantibodies?

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Multiple Choice

Which technique is used to reveal true ABO grouping in the presence of cold autoantibodies?

Cold autoantibodies interfere with ABO typing by binding to red cells at low temperatures, causing nonspecific agglutination that can mask the true ABO antigens on the patient’s cells. Cold autoabsorption and retest removes this interfering antibody. By adsorbing the autoantibody from the patient’s serum with donor red cells at cold temperature, the autoantibody is bound and washed away. When you retest, the reactions reflect the patient’s actual ABO antigens rather than the autoantibody effects, revealing the true ABO group. Warm autoabsorption targets autoantibodies that react at warmer temperatures (37°C) and is used when warm autoantibodies obscure alloantibody identification, not specifically to resolve cold autoantibody interference in ABO typing. Direct antiglobulin test detects whether antibodies are already bound to red cells in vivo, but it doesn’t correct the ABO typing interference. Enzyme treatment can modify antigen expression and alter reactivity, which may complicate typing rather than clarify it in this scenario.

Cold autoantibodies interfere with ABO typing by binding to red cells at low temperatures, causing nonspecific agglutination that can mask the true ABO antigens on the patient’s cells. Cold autoabsorption and retest removes this interfering antibody. By adsorbing the autoantibody from the patient’s serum with donor red cells at cold temperature, the autoantibody is bound and washed away. When you retest, the reactions reflect the patient’s actual ABO antigens rather than the autoantibody effects, revealing the true ABO group.

Warm autoabsorption targets autoantibodies that react at warmer temperatures (37°C) and is used when warm autoantibodies obscure alloantibody identification, not specifically to resolve cold autoantibody interference in ABO typing. Direct antiglobulin test detects whether antibodies are already bound to red cells in vivo, but it doesn’t correct the ABO typing interference. Enzyme treatment can modify antigen expression and alter reactivity, which may complicate typing rather than clarify it in this scenario.

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